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amplifier b1 molecular instruments custom probe against creert2  (Addgene inc)


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    Addgene inc amplifier b1 molecular instruments custom probe against creert2
    Figure 1. Genetic lineage tracing reveals that artery endothelial cells generate HSCs in vivo (A) Experimental strategy. DA, dorsal aorta; FL, fetal liver; PB, peripheral blood; BM, bone marrow; E, embryonic day; P, postnatal day. (B) Mass spectrometry quantification of (Z)-4OHT levels in plasma of female adult <t>Cx40-CreERT2</t> mice that intraperitoneally injected with (Z)-4OHT.
    Amplifier B1 Molecular Instruments Custom Probe Against Creert2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/amplifier+b1+molecular+instruments+custom+probe+against+creert2/pCAG-CreERT2+(Plasmid+%2314797)/pm38569552-236-200-212
    Average 95 stars, based on 96 article reviews
    amplifier b1 molecular instruments custom probe against creert2 - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Lineage-tracing hematopoietic stem cell origins in vivo to efficiently make human HLF+ HOXA+ hematopoietic progenitors from pluripotent stem cells."

    Article Title: Lineage-tracing hematopoietic stem cell origins in vivo to efficiently make human HLF+ HOXA+ hematopoietic progenitors from pluripotent stem cells.

    Journal: Developmental cell

    doi: 10.1016/j.devcel.2024.03.003

    Figure 1. Genetic lineage tracing reveals that artery endothelial cells generate HSCs in vivo (A) Experimental strategy. DA, dorsal aorta; FL, fetal liver; PB, peripheral blood; BM, bone marrow; E, embryonic day; P, postnatal day. (B) Mass spectrometry quantification of (Z)-4OHT levels in plasma of female adult Cx40-CreERT2 mice that intraperitoneally injected with (Z)-4OHT.
    Figure Legend Snippet: Figure 1. Genetic lineage tracing reveals that artery endothelial cells generate HSCs in vivo (A) Experimental strategy. DA, dorsal aorta; FL, fetal liver; PB, peripheral blood; BM, bone marrow; E, embryonic day; P, postnatal day. (B) Mass spectrometry quantification of (Z)-4OHT levels in plasma of female adult Cx40-CreERT2 mice that intraperitoneally injected with (Z)-4OHT.

    Techniques Used: In Vivo, Mass Spectrometry, Clinical Proteomics, Injection

    Figure 2. Artery-derived HSCs are functional in vivo (A–E) Arteries were lineage-traced in Cx40-CreERT2; Ai6 (ZsGreen reporter) embryos by administering 4OHT at either E8.0, E8.5, or E9.0. After embryos developed into adults, flow cytometry was performed to quantify ZsGreen+ cells in (B) and (C) peripheral blood and (C) and (D) bone marrow HSCs in 1- to 22-month-old adult mice. Line graphs depict the mean ± SEM. Related to Figure S3.
    Figure Legend Snippet: Figure 2. Artery-derived HSCs are functional in vivo (A–E) Arteries were lineage-traced in Cx40-CreERT2; Ai6 (ZsGreen reporter) embryos by administering 4OHT at either E8.0, E8.5, or E9.0. After embryos developed into adults, flow cytometry was performed to quantify ZsGreen+ cells in (B) and (C) peripheral blood and (C) and (D) bone marrow HSCs in 1- to 22-month-old adult mice. Line graphs depict the mean ± SEM. Related to Figure S3.

    Techniques Used: Derivative Assay, Functional Assay, In Vivo, Cytometry

    Related Articles

    Sequencing:

    Article Title: Lineage-tracing hematopoietic stem cell origins in vivo to efficiently make human HLF+ HOXA+ hematopoietic progenitors from pluripotent stem cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER H7 CAG-AkaLuc-PuroR hESCs Kyle Loh’s laboratory (Stanford University) This study WTC11 hiPSCs Coriell Institute for Medical Research Coriell Institute for Medical Research, GM25256, Kreitzer et al.148 OP9-DLL4-IRES-GFP feeder cells Juan Carlos Zúñiga-Pfl€ucker’s laboratory (University of Toronto) Mohtashami et al.149 MS5 feeder cells DSMZ ACC 441 Human cord blood CD34+ hematopoietic stem and progenitor cells (pooled from mixed donors) StemExpress CB3400.5C Experimental models: organisms/strains Mus musculus (mouse): Cx40-CreERT2 Lucile Miquerol’s laboratory (Aix-Marseille Université) Beyer et al.77 Mus musculus (mouse): Efnb2-CreERT2 Kyle Loh’s laboratory (Stanford University) This study (being deposited at The Jackson Laboratory [JAX], 038831) Mus musculus (mouse): Apj-CreERT2 Kristy Red-Horse’s laboratory (Stanford University) Chen et al.91 Mus musculus (mouse): Rosa26-CAG-LoxP-StopLoxP-ZsGreen (Ai6) The Jackson Laboratory JAX 007906 (Madisen et al.78) Mus musculus (mouse): FVB/NJ The Jackson Laboratory JAX 001800 Mus musculus (mouse): C57BL/6-CD45.2 The Jackson Laboratory JAX 000664 Mus musculus (mouse): C57BL/6-CD45.1 (Pepboy) The Jackson Laboratory JAX 002014 Mus musculus (mouse): NOD-SCID Il2rg-/- (NSG) The Jackson Laboratory JAX 005557 (Shultz et al.150) Oligonucleotides Primers See Table S4 N/A HCR3 probe for mouse Gja5 (Cx40), compatible with amplifier B3 Molecular Instruments Custom probe against Gja5 (sequence deposited at NCBI accession NM_001271628) HCR3 probe for CreERT2, compatible with amplifier B1 Molecular Instruments Custom probe against CreERT2 (sequence deposited at https:// www.addgene.org/14797/) HCR3 B3-Alexa Fluor 647 amplifier probe Molecular Instruments B3-Alexa Fluor 647 HCR3 B1-Alexa Fluor 488 amplifier probe Molecular Instruments B1-Alexa Fluor 488 Software and algorithms anndata Fabian Theis’s laboratory (Helmholtz Munich) https://github.com/ theislab/anndata AnnotationDbi Hervé Pagès, Marc Carlson, Seth Falcon, Nianhua Li (Bioconductor Team) https://github.com/ Bioconductor/ AnnotationDbi biomaRt Damian Smedley, Syed Haider, Benoit Ballester, Richard Holland, Darin London, Gudmundur Thorisson, Arek Kasprzyk (Bioconductor Team) https://github.com/ grimbough/biomaRt CellRanger 10x Genomics https://support.

    Software:

    Article Title: Lineage-tracing hematopoietic stem cell origins in vivo to efficiently make human HLF+ HOXA+ hematopoietic progenitors from pluripotent stem cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER H7 CAG-AkaLuc-PuroR hESCs Kyle Loh’s laboratory (Stanford University) This study WTC11 hiPSCs Coriell Institute for Medical Research Coriell Institute for Medical Research, GM25256, Kreitzer et al.148 OP9-DLL4-IRES-GFP feeder cells Juan Carlos Zúñiga-Pfl€ucker’s laboratory (University of Toronto) Mohtashami et al.149 MS5 feeder cells DSMZ ACC 441 Human cord blood CD34+ hematopoietic stem and progenitor cells (pooled from mixed donors) StemExpress CB3400.5C Experimental models: organisms/strains Mus musculus (mouse): Cx40-CreERT2 Lucile Miquerol’s laboratory (Aix-Marseille Université) Beyer et al.77 Mus musculus (mouse): Efnb2-CreERT2 Kyle Loh’s laboratory (Stanford University) This study (being deposited at The Jackson Laboratory [JAX], 038831) Mus musculus (mouse): Apj-CreERT2 Kristy Red-Horse’s laboratory (Stanford University) Chen et al.91 Mus musculus (mouse): Rosa26-CAG-LoxP-StopLoxP-ZsGreen (Ai6) The Jackson Laboratory JAX 007906 (Madisen et al.78) Mus musculus (mouse): FVB/NJ The Jackson Laboratory JAX 001800 Mus musculus (mouse): C57BL/6-CD45.2 The Jackson Laboratory JAX 000664 Mus musculus (mouse): C57BL/6-CD45.1 (Pepboy) The Jackson Laboratory JAX 002014 Mus musculus (mouse): NOD-SCID Il2rg-/- (NSG) The Jackson Laboratory JAX 005557 (Shultz et al.150) Oligonucleotides Primers See Table S4 N/A HCR3 probe for mouse Gja5 (Cx40), compatible with amplifier B3 Molecular Instruments Custom probe against Gja5 (sequence deposited at NCBI accession NM_001271628) HCR3 probe for CreERT2, compatible with amplifier B1 Molecular Instruments Custom probe against CreERT2 (sequence deposited at https:// www.addgene.org/14797/) HCR3 B3-Alexa Fluor 647 amplifier probe Molecular Instruments B3-Alexa Fluor 647 HCR3 B1-Alexa Fluor 488 amplifier probe Molecular Instruments B1-Alexa Fluor 488 Software and algorithms anndata Fabian Theis’s laboratory (Helmholtz Munich) https://github.com/ theislab/anndata AnnotationDbi Hervé Pagès, Marc Carlson, Seth Falcon, Nianhua Li (Bioconductor Team) https://github.com/ Bioconductor/ AnnotationDbi biomaRt Damian Smedley, Syed Haider, Benoit Ballester, Richard Holland, Darin London, Gudmundur Thorisson, Arek Kasprzyk (Bioconductor Team) https://github.com/ grimbough/biomaRt CellRanger 10x Genomics https://support.

    In Vivo:

    Article Title: Lineage-tracing hematopoietic stem cell origins in vivo to efficiently make human HLF+ HOXA+ hematopoietic progenitors from pluripotent stem cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER H7 CAG-AkaLuc-PuroR hESCs Kyle Loh’s laboratory (Stanford University) This study WTC11 hiPSCs Coriell Institute for Medical Research Coriell Institute for Medical Research, GM25256, Kreitzer et al.148 OP9-DLL4-IRES-GFP feeder cells Juan Carlos Zúñiga-Pfl€ucker’s laboratory (University of Toronto) Mohtashami et al.149 MS5 feeder cells DSMZ ACC 441 Human cord blood CD34+ hematopoietic stem and progenitor cells (pooled from mixed donors) StemExpress CB3400.5C Experimental models: organisms/strains Mus musculus (mouse): Cx40-CreERT2 Lucile Miquerol’s laboratory (Aix-Marseille Université) Beyer et al.77 Mus musculus (mouse): Efnb2-CreERT2 Kyle Loh’s laboratory (Stanford University) This study (being deposited at The Jackson Laboratory [JAX], 038831) Mus musculus (mouse): Apj-CreERT2 Kristy Red-Horse’s laboratory (Stanford University) Chen et al.91 Mus musculus (mouse): Rosa26-CAG-LoxP-StopLoxP-ZsGreen (Ai6) The Jackson Laboratory JAX 007906 (Madisen et al.78) Mus musculus (mouse): FVB/NJ The Jackson Laboratory JAX 001800 Mus musculus (mouse): C57BL/6-CD45.2 The Jackson Laboratory JAX 000664 Mus musculus (mouse): C57BL/6-CD45.1 (Pepboy) The Jackson Laboratory JAX 002014 Mus musculus (mouse): NOD-SCID Il2rg-/- (NSG) The Jackson Laboratory JAX 005557 (Shultz et al.150) Oligonucleotides Primers See Table S4 N/A HCR3 probe for mouse Gja5 (Cx40), compatible with amplifier B3 Molecular Instruments Custom probe against Gja5 (sequence deposited at NCBI accession NM_001271628) HCR3 probe for CreERT2, compatible with amplifier B1 Molecular Instruments Custom probe against CreERT2 (sequence deposited at https:// www.addgene.org/14797/) HCR3 B3-Alexa Fluor 647 amplifier probe Molecular Instruments B3-Alexa Fluor 647 HCR3 B1-Alexa Fluor 488 amplifier probe Molecular Instruments B1-Alexa Fluor 488 Software and algorithms anndata Fabian Theis’s laboratory (Helmholtz Munich) https://github.com/ theislab/anndata AnnotationDbi Hervé Pagès, Marc Carlson, Seth Falcon, Nianhua Li (Bioconductor Team) https://github.com/ Bioconductor/ AnnotationDbi biomaRt Damian Smedley, Syed Haider, Benoit Ballester, Richard Holland, Darin London, Gudmundur Thorisson, Arek Kasprzyk (Bioconductor Team) https://github.com/ grimbough/biomaRt CellRanger 10x Genomics https://support.

    Mass Spectrometry:

    Article Title: Lineage-tracing hematopoietic stem cell origins in vivo to efficiently make human HLF+ HOXA+ hematopoietic progenitors from pluripotent stem cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER H7 CAG-AkaLuc-PuroR hESCs Kyle Loh’s laboratory (Stanford University) This study WTC11 hiPSCs Coriell Institute for Medical Research Coriell Institute for Medical Research, GM25256, Kreitzer et al.148 OP9-DLL4-IRES-GFP feeder cells Juan Carlos Zúñiga-Pfl€ucker’s laboratory (University of Toronto) Mohtashami et al.149 MS5 feeder cells DSMZ ACC 441 Human cord blood CD34+ hematopoietic stem and progenitor cells (pooled from mixed donors) StemExpress CB3400.5C Experimental models: organisms/strains Mus musculus (mouse): Cx40-CreERT2 Lucile Miquerol’s laboratory (Aix-Marseille Université) Beyer et al.77 Mus musculus (mouse): Efnb2-CreERT2 Kyle Loh’s laboratory (Stanford University) This study (being deposited at The Jackson Laboratory [JAX], 038831) Mus musculus (mouse): Apj-CreERT2 Kristy Red-Horse’s laboratory (Stanford University) Chen et al.91 Mus musculus (mouse): Rosa26-CAG-LoxP-StopLoxP-ZsGreen (Ai6) The Jackson Laboratory JAX 007906 (Madisen et al.78) Mus musculus (mouse): FVB/NJ The Jackson Laboratory JAX 001800 Mus musculus (mouse): C57BL/6-CD45.2 The Jackson Laboratory JAX 000664 Mus musculus (mouse): C57BL/6-CD45.1 (Pepboy) The Jackson Laboratory JAX 002014 Mus musculus (mouse): NOD-SCID Il2rg-/- (NSG) The Jackson Laboratory JAX 005557 (Shultz et al.150) Oligonucleotides Primers See Table S4 N/A HCR3 probe for mouse Gja5 (Cx40), compatible with amplifier B3 Molecular Instruments Custom probe against Gja5 (sequence deposited at NCBI accession NM_001271628) HCR3 probe for CreERT2, compatible with amplifier B1 Molecular Instruments Custom probe against CreERT2 (sequence deposited at https:// www.addgene.org/14797/) HCR3 B3-Alexa Fluor 647 amplifier probe Molecular Instruments B3-Alexa Fluor 647 HCR3 B1-Alexa Fluor 488 amplifier probe Molecular Instruments B1-Alexa Fluor 488 Software and algorithms anndata Fabian Theis’s laboratory (Helmholtz Munich) https://github.com/ theislab/anndata AnnotationDbi Hervé Pagès, Marc Carlson, Seth Falcon, Nianhua Li (Bioconductor Team) https://github.com/ Bioconductor/ AnnotationDbi biomaRt Damian Smedley, Syed Haider, Benoit Ballester, Richard Holland, Darin London, Gudmundur Thorisson, Arek Kasprzyk (Bioconductor Team) https://github.com/ grimbough/biomaRt CellRanger 10x Genomics https://support.

    Clinical Proteomics:

    Article Title: Lineage-tracing hematopoietic stem cell origins in vivo to efficiently make human HLF+ HOXA+ hematopoietic progenitors from pluripotent stem cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER H7 CAG-AkaLuc-PuroR hESCs Kyle Loh’s laboratory (Stanford University) This study WTC11 hiPSCs Coriell Institute for Medical Research Coriell Institute for Medical Research, GM25256, Kreitzer et al.148 OP9-DLL4-IRES-GFP feeder cells Juan Carlos Zúñiga-Pfl€ucker’s laboratory (University of Toronto) Mohtashami et al.149 MS5 feeder cells DSMZ ACC 441 Human cord blood CD34+ hematopoietic stem and progenitor cells (pooled from mixed donors) StemExpress CB3400.5C Experimental models: organisms/strains Mus musculus (mouse): Cx40-CreERT2 Lucile Miquerol’s laboratory (Aix-Marseille Université) Beyer et al.77 Mus musculus (mouse): Efnb2-CreERT2 Kyle Loh’s laboratory (Stanford University) This study (being deposited at The Jackson Laboratory [JAX], 038831) Mus musculus (mouse): Apj-CreERT2 Kristy Red-Horse’s laboratory (Stanford University) Chen et al.91 Mus musculus (mouse): Rosa26-CAG-LoxP-StopLoxP-ZsGreen (Ai6) The Jackson Laboratory JAX 007906 (Madisen et al.78) Mus musculus (mouse): FVB/NJ The Jackson Laboratory JAX 001800 Mus musculus (mouse): C57BL/6-CD45.2 The Jackson Laboratory JAX 000664 Mus musculus (mouse): C57BL/6-CD45.1 (Pepboy) The Jackson Laboratory JAX 002014 Mus musculus (mouse): NOD-SCID Il2rg-/- (NSG) The Jackson Laboratory JAX 005557 (Shultz et al.150) Oligonucleotides Primers See Table S4 N/A HCR3 probe for mouse Gja5 (Cx40), compatible with amplifier B3 Molecular Instruments Custom probe against Gja5 (sequence deposited at NCBI accession NM_001271628) HCR3 probe for CreERT2, compatible with amplifier B1 Molecular Instruments Custom probe against CreERT2 (sequence deposited at https:// www.addgene.org/14797/) HCR3 B3-Alexa Fluor 647 amplifier probe Molecular Instruments B3-Alexa Fluor 647 HCR3 B1-Alexa Fluor 488 amplifier probe Molecular Instruments B1-Alexa Fluor 488 Software and algorithms anndata Fabian Theis’s laboratory (Helmholtz Munich) https://github.com/ theislab/anndata AnnotationDbi Hervé Pagès, Marc Carlson, Seth Falcon, Nianhua Li (Bioconductor Team) https://github.com/ Bioconductor/ AnnotationDbi biomaRt Damian Smedley, Syed Haider, Benoit Ballester, Richard Holland, Darin London, Gudmundur Thorisson, Arek Kasprzyk (Bioconductor Team) https://github.com/ grimbough/biomaRt CellRanger 10x Genomics https://support.

    Injection:

    Article Title: Lineage-tracing hematopoietic stem cell origins in vivo to efficiently make human HLF+ HOXA+ hematopoietic progenitors from pluripotent stem cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER H7 CAG-AkaLuc-PuroR hESCs Kyle Loh’s laboratory (Stanford University) This study WTC11 hiPSCs Coriell Institute for Medical Research Coriell Institute for Medical Research, GM25256, Kreitzer et al.148 OP9-DLL4-IRES-GFP feeder cells Juan Carlos Zúñiga-Pfl€ucker’s laboratory (University of Toronto) Mohtashami et al.149 MS5 feeder cells DSMZ ACC 441 Human cord blood CD34+ hematopoietic stem and progenitor cells (pooled from mixed donors) StemExpress CB3400.5C Experimental models: organisms/strains Mus musculus (mouse): Cx40-CreERT2 Lucile Miquerol’s laboratory (Aix-Marseille Université) Beyer et al.77 Mus musculus (mouse): Efnb2-CreERT2 Kyle Loh’s laboratory (Stanford University) This study (being deposited at The Jackson Laboratory [JAX], 038831) Mus musculus (mouse): Apj-CreERT2 Kristy Red-Horse’s laboratory (Stanford University) Chen et al.91 Mus musculus (mouse): Rosa26-CAG-LoxP-StopLoxP-ZsGreen (Ai6) The Jackson Laboratory JAX 007906 (Madisen et al.78) Mus musculus (mouse): FVB/NJ The Jackson Laboratory JAX 001800 Mus musculus (mouse): C57BL/6-CD45.2 The Jackson Laboratory JAX 000664 Mus musculus (mouse): C57BL/6-CD45.1 (Pepboy) The Jackson Laboratory JAX 002014 Mus musculus (mouse): NOD-SCID Il2rg-/- (NSG) The Jackson Laboratory JAX 005557 (Shultz et al.150) Oligonucleotides Primers See Table S4 N/A HCR3 probe for mouse Gja5 (Cx40), compatible with amplifier B3 Molecular Instruments Custom probe against Gja5 (sequence deposited at NCBI accession NM_001271628) HCR3 probe for CreERT2, compatible with amplifier B1 Molecular Instruments Custom probe against CreERT2 (sequence deposited at https:// www.addgene.org/14797/) HCR3 B3-Alexa Fluor 647 amplifier probe Molecular Instruments B3-Alexa Fluor 647 HCR3 B1-Alexa Fluor 488 amplifier probe Molecular Instruments B1-Alexa Fluor 488 Software and algorithms anndata Fabian Theis’s laboratory (Helmholtz Munich) https://github.com/ theislab/anndata AnnotationDbi Hervé Pagès, Marc Carlson, Seth Falcon, Nianhua Li (Bioconductor Team) https://github.com/ Bioconductor/ AnnotationDbi biomaRt Damian Smedley, Syed Haider, Benoit Ballester, Richard Holland, Darin London, Gudmundur Thorisson, Arek Kasprzyk (Bioconductor Team) https://github.com/ grimbough/biomaRt CellRanger 10x Genomics https://support.

    Derivative Assay:

    Article Title: Lineage-tracing hematopoietic stem cell origins in vivo to efficiently make human HLF+ HOXA+ hematopoietic progenitors from pluripotent stem cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER H7 CAG-AkaLuc-PuroR hESCs Kyle Loh’s laboratory (Stanford University) This study WTC11 hiPSCs Coriell Institute for Medical Research Coriell Institute for Medical Research, GM25256, Kreitzer et al.148 OP9-DLL4-IRES-GFP feeder cells Juan Carlos Zúñiga-Pfl€ucker’s laboratory (University of Toronto) Mohtashami et al.149 MS5 feeder cells DSMZ ACC 441 Human cord blood CD34+ hematopoietic stem and progenitor cells (pooled from mixed donors) StemExpress CB3400.5C Experimental models: organisms/strains Mus musculus (mouse): Cx40-CreERT2 Lucile Miquerol’s laboratory (Aix-Marseille Université) Beyer et al.77 Mus musculus (mouse): Efnb2-CreERT2 Kyle Loh’s laboratory (Stanford University) This study (being deposited at The Jackson Laboratory [JAX], 038831) Mus musculus (mouse): Apj-CreERT2 Kristy Red-Horse’s laboratory (Stanford University) Chen et al.91 Mus musculus (mouse): Rosa26-CAG-LoxP-StopLoxP-ZsGreen (Ai6) The Jackson Laboratory JAX 007906 (Madisen et al.78) Mus musculus (mouse): FVB/NJ The Jackson Laboratory JAX 001800 Mus musculus (mouse): C57BL/6-CD45.2 The Jackson Laboratory JAX 000664 Mus musculus (mouse): C57BL/6-CD45.1 (Pepboy) The Jackson Laboratory JAX 002014 Mus musculus (mouse): NOD-SCID Il2rg-/- (NSG) The Jackson Laboratory JAX 005557 (Shultz et al.150) Oligonucleotides Primers See Table S4 N/A HCR3 probe for mouse Gja5 (Cx40), compatible with amplifier B3 Molecular Instruments Custom probe against Gja5 (sequence deposited at NCBI accession NM_001271628) HCR3 probe for CreERT2, compatible with amplifier B1 Molecular Instruments Custom probe against CreERT2 (sequence deposited at https:// www.addgene.org/14797/) HCR3 B3-Alexa Fluor 647 amplifier probe Molecular Instruments B3-Alexa Fluor 647 HCR3 B1-Alexa Fluor 488 amplifier probe Molecular Instruments B1-Alexa Fluor 488 Software and algorithms anndata Fabian Theis’s laboratory (Helmholtz Munich) https://github.com/ theislab/anndata AnnotationDbi Hervé Pagès, Marc Carlson, Seth Falcon, Nianhua Li (Bioconductor Team) https://github.com/ Bioconductor/ AnnotationDbi biomaRt Damian Smedley, Syed Haider, Benoit Ballester, Richard Holland, Darin London, Gudmundur Thorisson, Arek Kasprzyk (Bioconductor Team) https://github.com/ grimbough/biomaRt CellRanger 10x Genomics https://support.

    Functional Assay:

    Article Title: Lineage-tracing hematopoietic stem cell origins in vivo to efficiently make human HLF+ HOXA+ hematopoietic progenitors from pluripotent stem cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER H7 CAG-AkaLuc-PuroR hESCs Kyle Loh’s laboratory (Stanford University) This study WTC11 hiPSCs Coriell Institute for Medical Research Coriell Institute for Medical Research, GM25256, Kreitzer et al.148 OP9-DLL4-IRES-GFP feeder cells Juan Carlos Zúñiga-Pfl€ucker’s laboratory (University of Toronto) Mohtashami et al.149 MS5 feeder cells DSMZ ACC 441 Human cord blood CD34+ hematopoietic stem and progenitor cells (pooled from mixed donors) StemExpress CB3400.5C Experimental models: organisms/strains Mus musculus (mouse): Cx40-CreERT2 Lucile Miquerol’s laboratory (Aix-Marseille Université) Beyer et al.77 Mus musculus (mouse): Efnb2-CreERT2 Kyle Loh’s laboratory (Stanford University) This study (being deposited at The Jackson Laboratory [JAX], 038831) Mus musculus (mouse): Apj-CreERT2 Kristy Red-Horse’s laboratory (Stanford University) Chen et al.91 Mus musculus (mouse): Rosa26-CAG-LoxP-StopLoxP-ZsGreen (Ai6) The Jackson Laboratory JAX 007906 (Madisen et al.78) Mus musculus (mouse): FVB/NJ The Jackson Laboratory JAX 001800 Mus musculus (mouse): C57BL/6-CD45.2 The Jackson Laboratory JAX 000664 Mus musculus (mouse): C57BL/6-CD45.1 (Pepboy) The Jackson Laboratory JAX 002014 Mus musculus (mouse): NOD-SCID Il2rg-/- (NSG) The Jackson Laboratory JAX 005557 (Shultz et al.150) Oligonucleotides Primers See Table S4 N/A HCR3 probe for mouse Gja5 (Cx40), compatible with amplifier B3 Molecular Instruments Custom probe against Gja5 (sequence deposited at NCBI accession NM_001271628) HCR3 probe for CreERT2, compatible with amplifier B1 Molecular Instruments Custom probe against CreERT2 (sequence deposited at https:// www.addgene.org/14797/) HCR3 B3-Alexa Fluor 647 amplifier probe Molecular Instruments B3-Alexa Fluor 647 HCR3 B1-Alexa Fluor 488 amplifier probe Molecular Instruments B1-Alexa Fluor 488 Software and algorithms anndata Fabian Theis’s laboratory (Helmholtz Munich) https://github.com/ theislab/anndata AnnotationDbi Hervé Pagès, Marc Carlson, Seth Falcon, Nianhua Li (Bioconductor Team) https://github.com/ Bioconductor/ AnnotationDbi biomaRt Damian Smedley, Syed Haider, Benoit Ballester, Richard Holland, Darin London, Gudmundur Thorisson, Arek Kasprzyk (Bioconductor Team) https://github.com/ grimbough/biomaRt CellRanger 10x Genomics https://support.

    Cytometry:

    Article Title: Lineage-tracing hematopoietic stem cell origins in vivo to efficiently make human HLF+ HOXA+ hematopoietic progenitors from pluripotent stem cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER H7 CAG-AkaLuc-PuroR hESCs Kyle Loh’s laboratory (Stanford University) This study WTC11 hiPSCs Coriell Institute for Medical Research Coriell Institute for Medical Research, GM25256, Kreitzer et al.148 OP9-DLL4-IRES-GFP feeder cells Juan Carlos Zúñiga-Pfl€ucker’s laboratory (University of Toronto) Mohtashami et al.149 MS5 feeder cells DSMZ ACC 441 Human cord blood CD34+ hematopoietic stem and progenitor cells (pooled from mixed donors) StemExpress CB3400.5C Experimental models: organisms/strains Mus musculus (mouse): Cx40-CreERT2 Lucile Miquerol’s laboratory (Aix-Marseille Université) Beyer et al.77 Mus musculus (mouse): Efnb2-CreERT2 Kyle Loh’s laboratory (Stanford University) This study (being deposited at The Jackson Laboratory [JAX], 038831) Mus musculus (mouse): Apj-CreERT2 Kristy Red-Horse’s laboratory (Stanford University) Chen et al.91 Mus musculus (mouse): Rosa26-CAG-LoxP-StopLoxP-ZsGreen (Ai6) The Jackson Laboratory JAX 007906 (Madisen et al.78) Mus musculus (mouse): FVB/NJ The Jackson Laboratory JAX 001800 Mus musculus (mouse): C57BL/6-CD45.2 The Jackson Laboratory JAX 000664 Mus musculus (mouse): C57BL/6-CD45.1 (Pepboy) The Jackson Laboratory JAX 002014 Mus musculus (mouse): NOD-SCID Il2rg-/- (NSG) The Jackson Laboratory JAX 005557 (Shultz et al.150) Oligonucleotides Primers See Table S4 N/A HCR3 probe for mouse Gja5 (Cx40), compatible with amplifier B3 Molecular Instruments Custom probe against Gja5 (sequence deposited at NCBI accession NM_001271628) HCR3 probe for CreERT2, compatible with amplifier B1 Molecular Instruments Custom probe against CreERT2 (sequence deposited at https:// www.addgene.org/14797/) HCR3 B3-Alexa Fluor 647 amplifier probe Molecular Instruments B3-Alexa Fluor 647 HCR3 B1-Alexa Fluor 488 amplifier probe Molecular Instruments B1-Alexa Fluor 488 Software and algorithms anndata Fabian Theis’s laboratory (Helmholtz Munich) https://github.com/ theislab/anndata AnnotationDbi Hervé Pagès, Marc Carlson, Seth Falcon, Nianhua Li (Bioconductor Team) https://github.com/ Bioconductor/ AnnotationDbi biomaRt Damian Smedley, Syed Haider, Benoit Ballester, Richard Holland, Darin London, Gudmundur Thorisson, Arek Kasprzyk (Bioconductor Team) https://github.com/ grimbough/biomaRt CellRanger 10x Genomics https://support.



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    amplifier b1 molecular instruments custom probe against creert2  (Addgene inc)
    95
    Addgene inc amplifier b1 molecular instruments custom probe against creert2
    Figure 1. Genetic lineage tracing reveals that artery endothelial cells generate HSCs in vivo (A) Experimental strategy. DA, dorsal aorta; FL, fetal liver; PB, peripheral blood; BM, bone marrow; E, embryonic day; P, postnatal day. (B) Mass spectrometry quantification of (Z)-4OHT levels in plasma of female adult <t>Cx40-CreERT2</t> mice that intraperitoneally injected with (Z)-4OHT.
    Amplifier B1 Molecular Instruments Custom Probe Against Creert2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/amplifier+b1+molecular+instruments+custom+probe+against+creert2/pCAG-CreERT2+(Plasmid+%2314797)/pm38569552-236-200-212
    Average 95 stars, based on 1 article reviews
    amplifier b1 molecular instruments custom probe against creert2 - by Bioz Stars, 2026-09
    95/100 stars
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    Image Search Results


    Figure 1. Genetic lineage tracing reveals that artery endothelial cells generate HSCs in vivo (A) Experimental strategy. DA, dorsal aorta; FL, fetal liver; PB, peripheral blood; BM, bone marrow; E, embryonic day; P, postnatal day. (B) Mass spectrometry quantification of (Z)-4OHT levels in plasma of female adult Cx40-CreERT2 mice that intraperitoneally injected with (Z)-4OHT.

    Journal: Developmental cell

    Article Title: Lineage-tracing hematopoietic stem cell origins in vivo to efficiently make human HLF+ HOXA+ hematopoietic progenitors from pluripotent stem cells.

    doi: 10.1016/j.devcel.2024.03.003

    Figure Lengend Snippet: Figure 1. Genetic lineage tracing reveals that artery endothelial cells generate HSCs in vivo (A) Experimental strategy. DA, dorsal aorta; FL, fetal liver; PB, peripheral blood; BM, bone marrow; E, embryonic day; P, postnatal day. (B) Mass spectrometry quantification of (Z)-4OHT levels in plasma of female adult Cx40-CreERT2 mice that intraperitoneally injected with (Z)-4OHT.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER H7 CAG-AkaLuc-PuroR hESCs Kyle Loh’s laboratory (Stanford University) This study WTC11 hiPSCs Coriell Institute for Medical Research Coriell Institute for Medical Research, GM25256, Kreitzer et al.148 OP9-DLL4-IRES-GFP feeder cells Juan Carlos Zúñiga-Pfl€ucker’s laboratory (University of Toronto) Mohtashami et al.149 MS5 feeder cells DSMZ ACC 441 Human cord blood CD34+ hematopoietic stem and progenitor cells (pooled from mixed donors) StemExpress CB3400.5C Experimental models: organisms/strains Mus musculus (mouse): Cx40-CreERT2 Lucile Miquerol’s laboratory (Aix-Marseille Université) Beyer et al.77 Mus musculus (mouse): Efnb2-CreERT2 Kyle Loh’s laboratory (Stanford University) This study (being deposited at The Jackson Laboratory [JAX], 038831) Mus musculus (mouse): Apj-CreERT2 Kristy Red-Horse’s laboratory (Stanford University) Chen et al.91 Mus musculus (mouse): Rosa26-CAG-LoxP-StopLoxP-ZsGreen (Ai6) The Jackson Laboratory JAX 007906 (Madisen et al.78) Mus musculus (mouse): FVB/NJ The Jackson Laboratory JAX 001800 Mus musculus (mouse): C57BL/6-CD45.2 The Jackson Laboratory JAX 000664 Mus musculus (mouse): C57BL/6-CD45.1 (Pepboy) The Jackson Laboratory JAX 002014 Mus musculus (mouse): NOD-SCID Il2rg-/- (NSG) The Jackson Laboratory JAX 005557 (Shultz et al.150) Oligonucleotides Primers See Table S4 N/A HCR3 probe for mouse Gja5 (Cx40), compatible with amplifier B3 Molecular Instruments Custom probe against Gja5 (sequence deposited at NCBI accession NM_001271628) HCR3 probe for CreERT2, compatible with amplifier B1 Molecular Instruments Custom probe against CreERT2 (sequence deposited at https:// www.addgene.org/14797/) HCR3 B3-Alexa Fluor 647 amplifier probe Molecular Instruments B3-Alexa Fluor 647 HCR3 B1-Alexa Fluor 488 amplifier probe Molecular Instruments B1-Alexa Fluor 488 Software and algorithms anndata Fabian Theis’s laboratory (Helmholtz Munich) https://github.com/ theislab/anndata AnnotationDbi Hervé Pagès, Marc Carlson, Seth Falcon, Nianhua Li (Bioconductor Team) https://github.com/ Bioconductor/ AnnotationDbi biomaRt Damian Smedley, Syed Haider, Benoit Ballester, Richard Holland, Darin London, Gudmundur Thorisson, Arek Kasprzyk (Bioconductor Team) https://github.com/ grimbough/biomaRt CellRanger 10x Genomics https://support.

    Techniques: In Vivo, Mass Spectrometry, Clinical Proteomics, Injection

    Figure 2. Artery-derived HSCs are functional in vivo (A–E) Arteries were lineage-traced in Cx40-CreERT2; Ai6 (ZsGreen reporter) embryos by administering 4OHT at either E8.0, E8.5, or E9.0. After embryos developed into adults, flow cytometry was performed to quantify ZsGreen+ cells in (B) and (C) peripheral blood and (C) and (D) bone marrow HSCs in 1- to 22-month-old adult mice. Line graphs depict the mean ± SEM. Related to Figure S3.

    Journal: Developmental cell

    Article Title: Lineage-tracing hematopoietic stem cell origins in vivo to efficiently make human HLF+ HOXA+ hematopoietic progenitors from pluripotent stem cells.

    doi: 10.1016/j.devcel.2024.03.003

    Figure Lengend Snippet: Figure 2. Artery-derived HSCs are functional in vivo (A–E) Arteries were lineage-traced in Cx40-CreERT2; Ai6 (ZsGreen reporter) embryos by administering 4OHT at either E8.0, E8.5, or E9.0. After embryos developed into adults, flow cytometry was performed to quantify ZsGreen+ cells in (B) and (C) peripheral blood and (C) and (D) bone marrow HSCs in 1- to 22-month-old adult mice. Line graphs depict the mean ± SEM. Related to Figure S3.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER H7 CAG-AkaLuc-PuroR hESCs Kyle Loh’s laboratory (Stanford University) This study WTC11 hiPSCs Coriell Institute for Medical Research Coriell Institute for Medical Research, GM25256, Kreitzer et al.148 OP9-DLL4-IRES-GFP feeder cells Juan Carlos Zúñiga-Pfl€ucker’s laboratory (University of Toronto) Mohtashami et al.149 MS5 feeder cells DSMZ ACC 441 Human cord blood CD34+ hematopoietic stem and progenitor cells (pooled from mixed donors) StemExpress CB3400.5C Experimental models: organisms/strains Mus musculus (mouse): Cx40-CreERT2 Lucile Miquerol’s laboratory (Aix-Marseille Université) Beyer et al.77 Mus musculus (mouse): Efnb2-CreERT2 Kyle Loh’s laboratory (Stanford University) This study (being deposited at The Jackson Laboratory [JAX], 038831) Mus musculus (mouse): Apj-CreERT2 Kristy Red-Horse’s laboratory (Stanford University) Chen et al.91 Mus musculus (mouse): Rosa26-CAG-LoxP-StopLoxP-ZsGreen (Ai6) The Jackson Laboratory JAX 007906 (Madisen et al.78) Mus musculus (mouse): FVB/NJ The Jackson Laboratory JAX 001800 Mus musculus (mouse): C57BL/6-CD45.2 The Jackson Laboratory JAX 000664 Mus musculus (mouse): C57BL/6-CD45.1 (Pepboy) The Jackson Laboratory JAX 002014 Mus musculus (mouse): NOD-SCID Il2rg-/- (NSG) The Jackson Laboratory JAX 005557 (Shultz et al.150) Oligonucleotides Primers See Table S4 N/A HCR3 probe for mouse Gja5 (Cx40), compatible with amplifier B3 Molecular Instruments Custom probe against Gja5 (sequence deposited at NCBI accession NM_001271628) HCR3 probe for CreERT2, compatible with amplifier B1 Molecular Instruments Custom probe against CreERT2 (sequence deposited at https:// www.addgene.org/14797/) HCR3 B3-Alexa Fluor 647 amplifier probe Molecular Instruments B3-Alexa Fluor 647 HCR3 B1-Alexa Fluor 488 amplifier probe Molecular Instruments B1-Alexa Fluor 488 Software and algorithms anndata Fabian Theis’s laboratory (Helmholtz Munich) https://github.com/ theislab/anndata AnnotationDbi Hervé Pagès, Marc Carlson, Seth Falcon, Nianhua Li (Bioconductor Team) https://github.com/ Bioconductor/ AnnotationDbi biomaRt Damian Smedley, Syed Haider, Benoit Ballester, Richard Holland, Darin London, Gudmundur Thorisson, Arek Kasprzyk (Bioconductor Team) https://github.com/ grimbough/biomaRt CellRanger 10x Genomics https://support.

    Techniques: Derivative Assay, Functional Assay, In Vivo, Cytometry